Western Blot Validation in Human Cell Lines
Loading: 15 ug of lysates per lane. Antibodies: PERP 56225 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide
Loading: 15 ug of lysates per lane. Antibodies: PERP 56225 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of PERP in A431 Cells
Immunocytochemical analysis of A431 cells using anti-PEPR antibody (56225) at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.